NeuroNET Research Center


UT Facilities

Advanced Microscopy and Imaging Center

The Center is a multi-user, multi-disciplinary facility that provides microscopy and imaging services in support of teaching and research for students, faculty and staff at UT. more information...

Contact:John Dunlap
101 Science and Engineering Research Facility
University of Tennessee
Knoxville, Tennessee 37996-0830
Phone: (865) 974-6197
Email: jrdunlap@utk.edu

  • Optical Microscopy
  • Transmission Electron Microscopy
  • Scanning Electron Microscopy and Focused Ion Beam
  • Atomic Force Microscopy
  • Sample Preparation Equipment
    • Dimpling grinder
    • Fischione plasma cleaner
    • Gatan Cryoplunge Cp3
    • Gatan precision ion polisher
    • Ladd critical point dryer
    • Leica EMFC7 ultramicrotome
    • Leica (Reichert) OMU3 ultramicrotome
    • Multi-prep polishing system
    • Slow speed diamond saw
    • SPI sputter coater

Molecular Biology Resource Facility

The MBRF is a self-supporting core facility specializing in the sequencing and analysis of DNA using Applied Biosystems' 3730 capillary electrophoresis DNA analyzer and Next Generation Sequencing using the Ion Torrent platform.  Our services now include Affymetrix gene expression analysis. more information...

Contact: Joseph A. May, Manager
Molecular Biology Resource Facility
A211 Walters Life Sciences Bldg.
University of Tennessee
Knoxville , TN 37996-0830
Phone: (865) 974-6231
Email: bioseq@utk.edu

  • Services Available:
    • Sanger Sequencing and Gene Scan Fragment Analysis
    • Next Gen Library Prep and Other Services
    • Affymetrix GeneChip Processing
    • Millipore Guava Easy Cyte 6HT-2L Flow Cytometer
    • Diagenode Bioruptor Sonication System UDC 300
    • Sage Science Pippin Prep
    • DNA Quantification
Instrument Application Type of use
Analytical Services    
     Atomic Spectroscopy Detect Elements Service
     Laser Diffraction Particle size measurement Service
Bioinformatics    
     Worksatation SeqMan NGen & CLC Assemble & analyze Sequences
Cellomics    
     Accuri Flow Cytometer Flow Cytometry Analyze samples
     Cellometer Vision Measure cell conc & Viability Brightfiels & Fluorescence
Genomics    
     CEQ8000 AFLP & RFLp Equipment usage
     Experion Quantify DNA, RNA & Proteins Equipment usage
     MyCycler PCR Equipment usage
     Nanodrop Quantify DNA, RNA & Proteins Equipment usage
     QIAxel Capillary electophoresis Equipment usage
     Qubit Quantify DNA, RNA & Proteins Equipment usage
Transcriptomics    
     ABI7900HT Fast Real Time PCR Equipment usage
     Maui Hyb Microarray Hyb station Equipment usage
     GenePix 4000 Microarray slide scanner Equipment usage
Proteomics    
     Luminex Immunoassay system Equipment usage
2D Gel Electrophoresis    
     IEF Isoelectric Focusing Equipment usage
     Protean Plus Dodeca Gel Electrophoresis Equipment usage
     Typhoon gel imager Visualize Fluorescent gels Equipment usage
     Ettan Spot picker Pick protein from gels Equipment usage
     BioTek Plate Reader Absorb,Fluor,Luminsc Equipment usage
Metabolics    
     HPLC PDA, RI detector, Fraction collection Equipment usage
Imaging    
     Phosphoimager 32P,33P,35S,14C,3H and 125I Equipment usage
Supporting Resources    
     Biomek 2000 Liquid handling robot Equipment usage
     Dishwasher Sterilization Clean glassware
     CO2 Tank Dry Ice Gas usage
     Liq. Nitrogen Tank   Gas usage

 

Computers for Next Generation Sequence Analysis

more information...

Dell Studio XPS 9000 Dell 7600
24 GB RAM 250 GB RAM
3 TB disk space 4 TB disk space
CLC Genomics NGen/DNaStar

Biological and Small Molecule Mass Spectrometry Core (BSMMSC)

Contact:
Dr. Shawn Campagna, Core Director
Department of Chemistry
University of Tennessee
618 Buehler Hall
Knoxville, TN 37996
Phone: 865-974-7337
Email: campagna@utk.edu

The Biological and Small Molecule Mass Spectrometry Core (BSMMSC) is housed in the department of chemistry at the University of Tennessee, Knoxville. It's located at Buehler Hall 613

Instrumentation

  • TSQ Quantum Discovery Max Triple Quadrupole Mass Spectometer
  • Exactive Quadrupole-Orbitrap Mass Spectrometer

Analyses and Rates

Type Manufacturer/Model Proposed FY 2015 Billing Rate
HPLC—Triple Quadrupole MS* Thermo/TSQ Quantum Discovery Max Assisted:
$18.00/hour Internal
$26.75/hour External Academic
Unassisted:
$5.00/hour Internal
$7.50/hour External Academic
HPLC—QTOF MS* Applied Biosystems/ QStar Elite Assisted:
$18.00/hour Internal
$26.75/hour External Academic
Unassisted:
$5.00/hour Internal
$7.50/hour External Academic
Without HPLC:
$10.50/hour Internal
$15.75/h External Academic
UPLC—Orbitrap MS* Thermo Scientific/ Exactive Plus Assisted:
$18.00/hour Internal
$26.75/hour External Academic
Unassisted:
$5.00/hour Internal
$7.50/hour External Academic
UPLC—Orbitrap MS* Thermo Scientific/ Exactive Plus Assisted:
$18.00/hour Internal
$26.75/hour External Academic
Unassisted:
$5.00/hour Internal
$7.50/hour External Academic
UPLC—Orbitrap MS* Thermo Scientific/ Exactive Plus Assisted:
$18.00/hour Internal
$26.75/hour External Academic
Unassisted:
$5.00/hour Internal
$7.50/hour External Academic
MALDI-TOF Applied Biosystems/ Voyager-DE PRO $10.50/hour Internal
$15.75/h External Academic
UPLC—Quadrupole-Orbitrap MS Thermo Scientific/ Q-Exactive Assisted:
$18.00/hour Internal
$26.75/hour External Academic
Unassisted:
$5.00/hour Internal
$7.50/hour External Academic

Bioanalytical Resource Facility

The BCMB department has access to a large collection of instrumentation for biophysical and biochemical characterization of proteins and nucleic acids under the heading of the Bioanalytical Resources Facility (BRF). more information...

Contact: Dr. Ed Wright, Facility Manager
Email: edwright@utk.edu

Instrumentation

  • Analytical Ultracentrifuge (AUC)
    A Beckman XL-I is utilized in the department to study proteins, peptides and nucleic acids. This technique is used to determine sedimentation coefficient, molar mass, purity and oligomeric state of a sample, as well as the stoichiometry and affinity of interactions.
  • Circular Dichroism (CD)
    An Aviv 202 CD spectrophotometer is used by researchers in the department to determine whether a protein is folded, characterize the global secondary structure of a protein and monitor folding and unfolding as a function of temperature, pH or denaturant concentration.
  • Differential Scanning Calorimeter (DSC)
    A VP-DSC is available to measure protein stability and energetics of unfolding and folding.
  • Electron Paramagnetic Resonance (EPR)
    Bruker EMX (X-band) Spectrometer is used to identify paramagnetic metals in a sample, quantify metal binding to a protein, study conformational changes and protein dynamics and monitor changes in the redox state of a system.
  • Fluorescence Spectroscopy
    A Perkin Elmer LS 55 to monitor protein folding, conformational changes, dynamics and binding interactions.
  • High Performance Liquid Chromatography (HPLC)
    An Agilent 1200 system is used in the department for separation of proteins, peptides and small molecules. This instrument is often used to prepare samples for mass spectrometry.
  • Isothermal Titration Calorimeters (ITC)
    Two VP-ITC instruments are used to measure affinity and thermodynamic parameters of interactions. Experiments evaluating protein-protein, protein-small molecule, protein-nucleic acid and protein-metal complexes are currently being performed in the department.
  • Mass Spectrometry
    A Bruker MALDI-TOF Mass Spectrometer is used in the department for accurate mass measurements of proteins and peptides
  • Surface Plasmon Resonance Spectrometer (SPR)
    A Biacore 3000 is used in the department to study biomolecular interactions. This technique not only measures binding affinity, but also quantifies the kinetics of the interaction. Binding events involving proteins, nucleic acids, carbohydrates and other small molecules are amenable to SPR analysis.
  • Rigaku R-axis IV diffractometer 
    Used for structure determination by X-ray crystallography.
  • Art Robbins Instruments Phoenix liquid handling system
    For automated screening of crystallization conditions.

NMR Facilities

more information...

Contact: Carlos A. Steren, Ph.D., Facilities Manager
321 Buehler Hall
Dept. of Chemistry
University of Tennessee
Knoxville, TN  37996-1600
Phone: (865) 974-3386
Email: steren@ion.chem.utk.edu

Instrumentation

  • Varian VNMRS 600 MHz
  • Varian VNMRS 500 MHz
  • Varian Inova 400 MHz
  • Bruker Avance 400 MHz
  • Varian Mercury 300 MHz/li>
  • Bruker AC 250 MHz

UT CCI-ORNL User-Experience Laboratory

Contact: Rachel Volentine, lab coordinator
Rm 230 Communication Bldg.
1345 Circle Park Drive
Knoxville, TN 39776
Email: rvolenti@utk.edu

The College of Communication and Information User-Experience Lab is the brainchild of a collaboration between the College and Oak Ridge National Laboratory (ORNL). The user-experience lab provides an environment conducive to studying how users interact with a system and conducive to gaining insight into the system's usability. For example, the lab can be used to conduct behavioral science-related studies measuring task performance and emotional reactions. The lab supports interdisciplinary research by UTK faculty members, graduate students, and ORNL researchers. Several Ph.D. students staff the user-experience lab while using the lab to support their own research agendas.

Physical Layout of the Lab

The lab is partitioned into three areas: (1) two participant rooms, which house the MORAE Recorder, (2) an observation room, which houses the MORAE Observer/Manager, and (3) a waiting lounge.

Participant Rooms

Experiments are conducted using the Recorders in the two participant rooms. Both rooms have the same hardware configurations, including a Microsoft Windows-based personal computer with dual monitors, web camera with a built-in microphone, a laser printer, and speakers. The web camera is used to capture the participants' facial expressions. The built-in microphone is designed to record participants' verbalizations as part of the thinking aloud protocol found in many studies. Also note the computers in the participant rooms are connected to the campus network.

Observation Room

The observation room is located between the two participant rooms. The observation room is equipped with two-way mirrors attached to the participant rooms to facilitate observations during experiments. The observation room is also equipped with a shared laser printer; speakers; and two Microsoft Windows-based computers, each of which can be connected to the participant rooms and to the Internet. In addition, the computers in the observation room have dual-monitor settings, speakers, and a shared laser printer.

Health Information Technology & Simulation Laboratory

Health Information Technology and Simulation Laboratory (HITS) is an organized research unit (ORU) at The University of Tennessee, Knoxville. It is built on collaborative research partnership between the College of Nursing and the College of Engineering and led by co-directors of Drs. Tami Wyatt and Xueping Li. The mission of the Health Information Technology and Simulation (HITS) ORU is to advance the science of health information technology and ways HITS enhance consumer health and professional health education.

HITS has more than 7,600 square feet of simulated healthcare space in which to provide a replica of the patient care environment where health care providers learn to apply cognitive, technical and psychomotor skills using an interdisciplinary approach.

The vision for the center is to instruct and facilitate learning that measures the learner's performance and competency in the health care environment and develops health care providers who continue to evaluate and apply principals learned in simulation to direct patient care.

For more information, see http://ilab.engr.utk.edu/hits/

Contact: Dr. Tami Wyatt
Email: twyatt@utk.edu

Institute of Biomedical Engineering (iBME)

The Institute of Biomedical Engineering (iBME) at the University of Tennessee has been established to research solutions to medical problems such as devices for improved delivery of medications and monitoring of patients; better imaging technology; regenerative models to help the body heal itself; and optimized efficiency in the healthcare setting. more information...

Contact: Institute of Biomedical Engineering (iBME)
311 Perkins Hall
1506 Middle Drive
Knoxville, TN 37996
Phone: (865) 974-7673
Fax: (865) 974-6394
info@ibme.utk.edu
http://ibme.utk.edu/about-us/equipment/

  • Accuri C6 Flow Cytometer
  • Aqueous GPC System
  • Biograph 6
  • Brice-Phoenix Differential Refractometer-Model BP-2000-V
  • Femtojet Microinjector and Micromanipulator
  • Inveon SPECT/PET/CT
  • Jupiter Vapor Pressure Osmometer
  • Knauer Membrane Osmometer
  • Leica SP2 LSCM
  • LEO 1525
  • LV EM5 Low Voltage Electron Microscope (SEM & TEM)
  • MFP-3D Atomic Force Microscope
  • MicroCAT II + SPECT Hybrid Scanner
  • Multimode Scanning Probe Microscope (SPM)
  • Nikon Eclipse E600
  • Nikon Ti
  • PDExpert Multi-Angle Light Scattering
  • Polymer Labs GPC120
  • Polymer Labs GPC220
  • PowerTome X Ultramicrotome
  • TA Instruments Q-1000 DSC
  • TA Instruments Q-50 TGA
  • TA Instruments Q-800 DMA
  • Tosoh EcoSEC GPC System
  • Uprint 3D Printer
  • ViscoSystems AVS 370 Dilute Solution Viscometer
  • Waters GPC System
  • Wyatt Technology Multi-Angle Light Scattering and Refractometer
  • Zeiss Auriga
  • Zeiss Libra 200 MC

Biology Service Facility

Contact: David E. Pratt.
Technical Director
125 Austin Peay Bldg.
University of Tennessee
Knoxville , TN 37996-0910
Phone: (865) 974-4219
Fax: (865) 974-0639
Email: depratt@utk.edu

Computer Services

The first point of contact within the Division of Biology for all computing issues.

Supported Operating Systems:

  • MS Windows

  • MAC OS

  • Linux

  • Unix

The service includes, but is not restricted to the following:

  • System Administration & Server Support
  • Specification of computer hardware.
  • Installing software.
  • Reloading Operating Systems.
  • Removing spyware and viruses.
  • Troubleshooting network problems.
  • Instrumentation computing issues.

Electronic Services

Maintenance of Laboratory Instrumentation

The service includes, but is not restricted to, the following list of instruments.

  • UV/VIS, IR, Fluorescence, AA spectrophotometry
  • Radiation counting
  • Ultra-centrifugation
  • Low-speed preparative centrifugation
  • Clinical centrifugation
  • Particle counting
  • Chromatography
  • General laboratory measurement instrumentation, e.g., pH, physiograph, recorder, etc.
  • Environmental maintenance instrumentation, e.g., growth chambers, incubators, waterbaths, shakers, etc.
  • Refrigeration, low temperature incubators, freezers and ultralow freezers.
  • General mechanical laboratory equipment, e.g., vortex, stir, hot plate, etc.
  • Autoclaves
  • Microscopes (technicians factory trained)
  • Balances (technicians factory trained)
  • Audio/visual (excluding office equipment)

Machine Services

Maintenance and Development of Laboratory Instrumentation

This service includes the following types of hardware:

  • Vacuum Pumps
  • Compressors
  • Autoclaves (mechanical systems)
  • Environmental maintenance instrumentation, e.g., growth chambers, incubators, waterbaths, shakers, etc. (mechanical systems)

Hardware Design and Development

BSF uses the following materials:

  • Most ferrous metals
  • 204 or lighter grade stainless steel
  • Most non-ferrous metals
  • Most rigid plastics materials

Wood working Services

General carpentry and cabinet services include the following:

  • General repair
  • Minor laboratory renovation
  • Laboratory furniture construction



 

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